Research skills

field

Field experience

  • Design and realization of a census study on Zosterops borbonicus populations in highlands from Réunion.
  • Capture, morphometric studies and tissue sampling on protected animals (endemic birds from Mascarenes, endangered sea turtles).
  • Overall a total of seven months of research in the field.
  • Diving: CMAS level 1.

Laboratory skills

  • Methods in molecular biology: DNA and RNA extraction, PCR, cloning, restriction, primer design, work with precious samples.
  • Preparation of Next Generation Sequencing (NGS) libraries and adaptation of RAD-seq protocol to HiSeq2000 chemistry.
  • Awareness of techniques for target enrichment and resequencing.

Analytical skills

  • Statistics (R)
  • Chromatograms interpretation (Sequencher, Geneious)
  • Microsatellites preliminary analyses (GeneMapper, Microchecker)
  • Population genetics/genomics softwares (GENEPOP, Arlequin, DNAsp, IMa, Migrate-n, MSMC, ARGWeaver, PopGenome, GenABEL…)
  • Phylogeny and datations (MrBayes, BEAST, RAxML)
  • Aproximate Bayesian Computation (ms, fastsimcoal, package abc in R)
  • Bioinformatics (UNIX, regular expressions, shell scripts, classical tools in NGS such as bwa, LastZ, velvet, Picard tools).
  • Niche modeling (Maxent, RandomForest package in R, extraction of environmental data, ENMTools).
  • Densities estimation (Distance)
  • Office, Adobe CS5.
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